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AIMS: To find the new microbial parameters explaining the soil fertility from the microbial community viewpoint.METHODS AND RESULTS: Fatty acid methyl ester (FAME) analysis and terminal-restriction fragment length polymorphism (T-RFLP) analysis were carried out using 16 differently treated plots from the same field that had been kept under different fertilizer management systems since 1984. It was found that organic fertilizer application had small impact, whereas chemical fertilizer application, especially ammonium-nitrogen fertilizer, had strong impact on microbial community structures. Principal component analysis was conducted based on soil chemical and physical parameters, crop yields, FAMEs and terminal-restriction fragments (T-RFs) to provide 10 FAMEs and 10 T-RFs showing strong relation with soil fertility.CONCLUSION: We defined these 10 FAMEs and 10 T-RFs as 'keystone' biological parameters explaining soil fertility in the soil.SIGNIFICANCE AND IMPACT OF THE STUDY: This is the first report on the FAMEs and T-RFs related to soil fertility.
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OBJECTIVE: The rate of infection following cat bites appears to be greater than that from dog bites. To study the clinical picture, complications Cheap Nike Free Run 2 and microbiology (in humans and cats), this prospective study was performed.METHODS: A prospective study with patients with clinical symptoms of infection due to cat bites from three emergency wards during two years in Stockholm, Sweden. Aerobic and anaerobic cultures from the wounds were performed as well as cultures from the biting cat's mouth.
The taste-modifying protein, miraculin, has the unusual property of modifying sour taste into sweet taste. The complete amino acid sequence of miraculin purified from miracle fruits by a newly developed method (Theerasilp, S., and Kurihara, Y. (1988) J.
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